Commercial formulations of saccharin, sucralose or aspartame were added to the drinking water of lean C57BI/6 mice fed on normal chow. Mice that drank only water, or solutions containing 100% glucose or sucrose were used as controls.
Saccharin was further investigated. C57BI/6 mice were fed a high-fat diet (HFD), while consuming 0.1mg/ml commercial saccharin or pure glucose (control). The doses of NAS corresponded to the FDA acceptable daily intake in humans, adjusted to mouse weights.
Results showed significant glucose intolerance in both lean and HFD-fed mice exposed to the artificial sweeteners, while the water, glucose and sucrose controls all showed comparable glucose tolerance curves.
Reference: Suez, Jotham et al. ‘Artificial Sweeteners Induce Glucose Intolerance By Altering The Gut Microbiota’. Nature (2014)